Hi @NurNina ,
Thanks, I finally reproduce your issue.
You must enter the latitude and longitude of all five waypoints before it shows the route.
As an alternative workaround, I add the condition before each waypoint to check if the text box is blank. Please try this code:
"https://dev.virtualearth.net/REST/v1/Imagery/Map/Road/Routes?"&
If(!IsBlank(Seq1_Lat_1.Text)&&!IsBlank(Seq1_Long_1.Text),"wp.0="& Seq1_Lat_1.Text & "," & Seq1_Long_1.Text & ";66;1&") &
If(!IsBlank(Seq2_Lat_1.Text)&&!IsBlank(Seq2_Long_1.Text),"wp.1=" & Seq2_Lat_1.Text & "," & Seq2_Long_1.Text & ";66;2&") &
If(!IsBlank(Seq3_Lat_1.Text)&&!IsBlank(Seq3_Long_1.Text),"wp.2=" & Seq3_Lat_1.Text & "," & Seq3_Long_1.Text & ";66;3&") &
If(!IsBlank(Seq4_Lat_1.Text)&&!IsBlank(Seq4_Long_1.Text),"wp.3=" & Seq4_Lat_1.Text & "," & Seq4_Long_1.Text & ";66;4&") &
If(!IsBlank(Seq5_Lat_1.Text)&&!IsBlank(Seq5_Long_1.Text),"wp.4=" & Seq5_Lat_1.Text & "," & Seq5_Long_1.Text & ";66;5&") &
"key="&txtBingMapsKey_2.Text
Test Result:

Sik